NGS, also known as high-throughput sequencing, is a DNA sequencing technology which has revolutionized genomic and molecular research and has influenced all fields of biological research, it has become increasingly prevalent in modern society. NGS allows us to sequence DNA and RNA much more accurately, quickly and cheaply.

Library sequencing is a technique that encompasses the initial step of attaching adapters, which include elements like index sequences, barcodes, and sequencing primer binding regions, to both ends of the desired target fragments. Subsequently, these prepared fragments are subjected to sequencing using a dedicated sequencer. In contrast, targeted sequencing is characterized by the selective capture of particular genes or regions of interest before the sequencing phase. Notably, the principal methods for executing targeted sequencing comprise hybrid capture and multiplex PCR.