The PacBio Sequel sequencer is Pacific BioSciences' latest third-generation sequencing platform, utilizing Single-Molecule Real-Time (SMRT) sequencing technology. It boasts an average read length of up to 15 kilobases (Kb), with the longest reads extending to 40-60 Kb. The sequencing system comprises 16 SMRT cell sequencing modules, each capable of producing 5-10 gigabases (Gb) of data. Notably, SMRT technology allows for the sequencing of individual DNA molecules without the need for amplification, effectively mitigating PCR-induced GC bias and ensuring comprehensive and uniform sequence coverage. Presently, PacBio SMRT sequencing technology finds widespread applications in complex genome assembly and full-length transcriptome sequencing of eukaryotic organisms.
