Animal and Plant Whole Exome Sequencing (WES) is a targeted sequencing approach that focuses on the protein-coding regions (exons) of the genome. Since these regions contain many functionally important genetic variants, exome sequencing provides an efficient and cost-effective alternative to whole genome sequencing for trait discovery and genetic analysis.

Widely used in agriculture, breeding, genetics, and evolutionary studies, exome sequencing helps identify variants linked to important traits such as disease resistance, productivity, adaptation, and species diversity. It also supports conservation research by assessing genetic diversity in wildlife and endangered species.

Animal & Plant Whole Exome Capture Panels

N2Jenomics Lab Pvt. Ltd. offers exome sequencing solutions for a wide range of plant and animal species using advanced hybrid capture technology and next-generation sequencing (NGS).

Why Choose Our Animal & Plant Exome Sequencing Service?

• High Target Specificity

Accurate enrichment of coding regions or customized genomic targets for reliable variant detection.

• High Sequencing Accuracy

Deep sequencing coverage enables confident identification of SNPs, InDels, and other functional variants.

• Fast & Efficient Workflow

Advanced NGS platforms deliver high-quality data with rapid turnaround times.

• Cost-Effective Solution

By sequencing only coding regions, exome sequencing reduces overall sequencing costs while maximizing biological insights.

Applications

• Plant Research

• - Crop improvement and molecular breeding
• - Detection of genes associated with yield, quality, and disease resistance
• - QTL mapping and marker-assisted selection
• - Analysis of complex plant genomes
• - Microexon and functional genomics studies

• Animal Research

• - Disease genetics and mutation discovery
• - Livestock breeding and trait improvement
• - Population genetics and evolutionary studies
• - Functional genomics and comparative genomics
• - Microexon and gene regulation research

Animal & Plant Exome Sequencing Workflow

Our standardized workflow includes:

DNA Extraction → Library Preparation → Exome Capture → Target Enrichment → High-Throughput Sequencing → Data Quality Control → Read Alignment → Variant Detection → Functional Annotation & Reporting

Service Specifications

	Sample Requirements   Sample Requirements: Sample type Genomic DNA, Whole blood, Buccal swab, and Saliva of animal. Sample requirement ≥ 2 μg Sample concentration ≥ 30 ng/μL Sample purity OD 260/280 = 1.8-2.0
	Sequencing HiSeq platform PE150, MGI DNBSEQ-T7/DNBSEQ-G400. Standard sequencing coverage ≥ 50X; cancer sample ≥ 100X. More SNPs can be gained by increasing the coverage.
	Bioinformatics Analysis   We provide multiple customized bioinformatics analyses: Data Quality Control Variant Detection Variant Annotation Enrichment Analysis Genotype-Phenotype Association Analysis Population Genetics Analysis Evolutionary Analysis Note: Recommended data outputs and analysis contents displayed are for reference only. For detailed information, please contact us with your customized requests.

Analysis Pipeline

Deliverables

• • The original sequencing data

• • Experimental results
• • Data analysis report
• • Details in Whole Exome Sequencing for your writing (customization)

1. How do I choose the right exome sequencing platform?

Platform selection depends on your species, genome complexity, target region size, sequencing depth, and research objectives. Our experts help you select the most suitable capture strategy and sequencing platform for your project.

2. What are the latest trends in plant and animal exome sequencing?

Recent advances include improved capture technologies, lower sequencing costs, AI-assisted data analysis, and comparative genomics across multiple species, enabling faster and more comprehensive genetic studies.

3. Why choose exome sequencing instead of whole genome sequencing?

Exome sequencing targets the protein-coding regions of the genome, where most functional genetic variants occur. It provides a cost-effective solution while delivering high-quality data for gene discovery, trait analysis, and mutation identification.

4. How is exome sequencing data analyzed?

Our bioinformatics workflow includes read quality assessment, alignment to the reference genome, variant calling (SNPs and InDels), functional annotation, and comprehensive reporting to identify biologically significant genetic variants.